Continuous axenic cultivation of Pneumocystis carinii
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چکیده
منابع مشابه
Continuous axenic cultivation of Pneumocystis carinii.
Continuous axenic culture of Pneumocystis carinii has been achieved. A culture vessel is used that allows for frequent medium exchange without disturbance of organisms that grow attached to a collagen-coated porous membrane. The growth medium is based on Minimal Essential Medium with Earle's salt supplemented with S-adenosyl-L-methionine, putrescine, ferric pyrophosphate, N-acetyl glucosamine, ...
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SUMMARY In this research, which was done for two years, we investigated various liquid and solid media without feeder ceells, for the ability to support growth of P. carinii. Data obtained from counting the organisms nuclei showed that, addition of Cycloheximide, penicillin, & streptomycin to neopeptoneN-Acetyle Glucoseamine (N.P.G. medium) can provides growth requirements of organism in its...
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Experimental infections with visceral Leishmania spp. are frequently performed referring to stationary parasite cultures that are comprised of a mixture of metacyclic and non-metacyclic parasites often with little regard to time of culture and metacyclic purification. This may lead to misleading or irreproducible experimental data. It is known that the maintenance of Leishmania spp. in vitro re...
متن کاملPneumocystis carinii.
Although Giemsa staining has been routinely used for the detection of trophozoites and intracystic bodies in smears of bronchoalveolar lavage fluid (BAL) from patients with Pneumocystis cariniipneumonia, it does not normally stain the cyst wall. For detection ofthe cysts other stains such as toluidine Blue 'O' and methenamine silver must be used as well. Sulphation of smears before staining wit...
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The entity of interstitial plasma cell pneumonia due to Pneumocystis carinii has received increasing attention during the past 10 years. Extensive studies and reviews concerning this problem, its epidemiology and pathogenesis (Ahvenainen, 1957; Bachmann, 1954; Cahalane, 1962; Jirovec, 1 960a and b; Muller and Vietor, 1957), its clinical course and picture (Berdnikoff, 1959; Dvorak and Jirovec, ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1999
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.96.5.2402